Quinine Attenuates Cigarette Smoke Extract Induced Mucosal Inflammation and Oxidative Stress in the Zebrafish Model

Abstract: Introduction: Smoking is the primary cause of Chronic obstructive pulmonary disease (COPD) leading to alarming increase in worldwide death. Cigarette smoking a dangerous risk factor causing mucosal inflammation and lung damage caused by nicotine, which more addictive than other narcotics. This study was undertaken assess potential quinine ameliorating cigarette smoke extract (CSE) induced Zebrafish model. Materials Methods: CSE extracted water exposed concentrations ranging from 0.2 1.0 cig/L. IC50 found be 0.3 cig/L this concentration used for further studies. Induced were treated with 5 mg, 10mg 15 mg/kg analysed reactive oxygen species, nitric oxide levels, myeloperoxidase activity, histopathology expression pro-inflammatory (IL-1) anti-inflammatory (IL-10) cytokine. Results: The results indicate that exposure has significantly increased levels ROS, MPO, NO Upon treatment quinine, dose dependent decrease these observed higher 15mg/kg animals 8 days. cytokine, IL-10 tend increasing quinine. histopathological examination shown damaged gill filaments group Gills neutrophil accumulation whereas changes are not quinine-treated animals. Conclusion: Cumulatively, obtained confirm role CSE-induced Keywords: extracts, Mucosal inflammation, Quinine, Zebrafish.